Detection of Chlamydia pneumoniae and Chlamydia psittaci in sputum samples by PCR.
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چکیده
منابع مشابه
Detection of Chlamydia pneumoniae and Chlamydia psittaci in sputum samples by PCR.
AIMS To use the polymerase chain reaction (PCR) to detect Chlamydia pneumoniae and Chlamydia psittaci in sputum samples. METHODS A nested PCR was developed, the first stage of which amplified DNA from both C pneumoniae and C psittaci while the second stage targeted specifically at C pneumoniae, allowing the two species to be differentiated. The primers were designed not to amplify sequences f...
متن کاملSIMULTANEOUS DETECTION OF CHLAMYDIA PNEUlvlONIAE AND MYCOPLASMA PNEUMONIAE BY PCR
Both C. pneumoniae and M. pneumoniae are common causes of respiratory tract infection. At present, both are still diagnosed in the laboratory retrospectively by serology. This is despite many publications which indicate that PCR, which is not retrospective, is extremely good at detecting these organisms. We thought that a single PCR test which could detect both organisms simultaneously in a...
متن کاملMultiplex polymerase chain reaction for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia psittaci in respiratory samples.
AIMS To develop a multiplex polymerase chain reaction (PCR) for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia psittaci in respiratory samples. METHODS Oligonucleotide primers for the amplification of the DNA of these three organisms were optimised for use in combination in the same reaction. PCR products were detected by hybridisation with pooled int...
متن کاملPapers Multiplex polymerase chain reaction for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia psittaci in respiratory samples
Aims—To develop a multiplex polymerase chain reaction (PCR) for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chlamydia psittaci in respiratory samples. Methods—Oligonucleotide primers for the amplification of the DNA of these three organisms were optimised for use in combination in the same reaction. PCR products were detected by hybridisation with pooled inter...
متن کاملsimultaneous detection of chlamydia pneulvloniae and mycoplasma pneumoniae by pcr
both c. pneumoniae and m. pneumoniae are common causes of respiratory tract infection. at present, both are still diagnosed in the laboratory retrospectively by serology. this is despite many publications which indicate that pcr, which is not retrospective, is extremely good at detecting these organisms. we thought that a single pcr test which could detect both organisms simultaneously in a rou...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1993
ISSN: 0021-9746
DOI: 10.1136/jcp.46.4.313